Composite

Part:BBa_K2353000

Designed by: Julia Leveille, Lauren Hong, Emily Gibson, Gaurav Byagathvalli, Katie Barr, Alyssa Franklin, Christina Lee, Ellie Kim, Kevin Li, Megan Hong, Natalie Shih, Nithik Balachandran, Noora Chandasir, David S   Group: iGEM17_Lambert_GA   (2017-10-17)


tsPurple

tsPurple is composed of a ribosomal binding site and tsPurple (a purple chromoprotein which functions as a reporter). tsPurple is intended to be assembled with pLambdaR-LacI (BBa_K1911000),which is the promoter and regulatory system, and pLac-ClpXP-CI (BBa_K1911001),which is the non-lysosomal protease. The terminator is located at the beginning of pLac-ClpXP-CI. When fully assembled, p-lambda-r LacI transcribes tsPurple and LacI represses pLac, preventing transcription of ClpXP-CI. Upon induction with IPTG, LacI binds to IPTG which prevents repression of pLac. Therefore, ClpXP-CI is transcribed; theoretically this should not change the levels of expression of tsPurple since there is no degradation tag attached. The levels of expression of tsPurple with degradation tags can then be compared to the level of tsPurple expression (as a base-line comparison).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None